The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies [1]. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK Tcells. Primary rat splenocytes (RS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.

RS from ScienCell Research Laboratories are isolated from normal ***** rat spleen. RS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. RS are quality control tested for viability. RS are negative for mycoplasma, bacteria, yeast, and fungi. RS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and?are not intended for long-term culture.